3D Cell Expansion System

The 3D Cell Expansion System (3D-CES) is an integrated system designed for large scale expansion of anchorage-dependent cells in a 3-dimensional (3D) microenvironment. Culture and expansion of a large quantity of cells by the traditional two dimensional (2D) method is difficult and known to have many challenges. The 3D-CES is designed to lift this inherent limitation of 2D cell culture and expansion methods. With the 3D-CES, cells are grown and expanded on 3D polystyrene (PS) scaffolds. The unique dynamic system circulates media throughout the system using a peristaltic pump providing an efficient exchange of nutrients and waste between media and the cells. With minimum hands-on time, this innovative technology is more efficient and significantly reduces the amount of time, space, and labor needed to achieve the desired number of cells in a shorter amount of time compared to other methods.

Culture Chamber and Media Container

• The Culture Chamber and Media Container are placed inside a custom-made incubator which provides tubing connection through its side wall to the pump
• Culture Chamber can hold up to 15 pieces of polystyrene (PS) scaffolds
• Cells are seeded and expanded in the PS scaffold which provides a 3D micro-environment
• The capacity of cells for each culture chamber is about 200 - 300 million
• The Media Container can hold up to 3.5 liter
• For 200 - 300 million of cells, about 2.5 liter of media is needed
• Flow of media is from the Media Container in upward direction to the Culture Chamber through the PS scaffolds